For the operation of the division of marine fisheries; provided, that the division may expend funds for the Annisquam river marine research laboratory, marine research programs, a commercial fisheries program, a shellfish management program, including coastal area classification, mapping and technical assistance, and a shellfish classification program; provided further, that funds shall be expended on a recreational fisheries program to be reimbursed by federal funds; provided further, that the division shall continue to develop strategies to improve federal regulations governing the commercial fishing industry and to promote sustainable fisheries; provided further, that not less than $150,000 shall be expended for shellfish propagation in Barnstable, Dukes and Nantucket counties to be administered jointly by the director of Marine Fisheries and the aforementioned counties; provided further, that not less than $50,000 shall be expended for a green crab trapping program in the towns of Provincetown, Truro, Wellfleet, Eastham, Orleans, Chatham and Harwich to be distributed by the department of marine fisheries; and provided further, that not less than $575,000 shall be expended for a program of collaborative research by the division of marine fisheries through the Marine Fisheries Institute, in collaboration with the School for Marine Science and Technology at the University of Massachusetts at Dartmouth, that applies innovative technology to assess the biomass of fish in the region managed by the New England Fishery Management Council and hydrodynamic modeling of coastal waters to accurately assess delineation of shellfish growing area classifications and other applications to improve management of marine fisheries resources, and whelk fishery research to evaluate alternative management strategies; provided further, that not less than $175,000 shall be expended to the University of Massachusetts at Amherst for its Gloucester Marine Station in the city of Gloucester to support marsh restoration and revitalization activities including, but not limited to: (i) a green crab trapping program for applied research and product development; (ii) high-resolution drone mapping to assess deterioration of the Great Marsh; (iii) microplastic and macroplastic sampling; and (iv) an academic study and processing of samples from the water column, beach environment, marsh peat and eelgrass; provided further, that not less than $150,000 shall be expended to Center for Coastal Studies, Inc. for field operations and staffing to respond to entanglements of marine mammals and sea turtles in the coastal waters; and provided further, that not less than $195,000 shall be expended for water testing capacity improvements at the division including, but not limited to, personnel and supplies, in support of the shellfish and aquaculture industries in the south coast region of the commonwealth
