For the operation of the division of marine fisheries; provided, that the division may expend funds for the Annisquam river marine research laboratory, marine research programs, a commercial fisheries program, a shellfish management program, including coastal area classification, mapping and technical assistance and a shellfish classification program; provided further, that funds shall be expended on a recreational fisheries program to be reimbursed by federal funds; provided further, that the division shall continue to develop strategies to improve federal regulations governing the commercial fishing industry and to promote sustainable fisheries; provided further, that not less than $200,000 shall be expended for Gloucester Marine Genomics Institute Incorporated for the development and implementation of environmental DNA research and testing; provided further, that not less than $25,000 shall be expended for a Cape Cod green crab trapping program; provided further, that not less than $575,000 shall be expended for a program of collaborative research by the division of marine fisheries through the Marine Fisheries Institute, in collaboration with the School for Marine Science and Technology at the University of Massachusetts at Dartmouth, that applies innovative technology to assess the biomass of fish in the region managed by the New England Fishery Management Council and hydrodynamic modeling of coastal waters to accurately assess delineation of shellfish growing area classifications and other applications to improve management of marine fisheries resources, and whelk fishery research to evaluate alternative management strategies; provided further, that not less than $30,000 shall be expended to the Cape Cod Commercial Fishermen's Alliance, Inc. for a study to assess opportunities to expand the kelp industry; provided further, that not less than $150,000 shall be expended for shellfish propagation in the counties of Barnstable, Dukes County and Nantucket to be administered jointly by the director of the division of marine fisheries and the aforementioned counties; provided further, that not less than $175,000 shall be expended for marsh restoration and revitalization including, but not limited to: (i) a green crab trapping program for applied research and product development; (ii) high--resolution drone mapping of deterioration of the Great marsh; (iii) the continuation of microplastic and macroplastic sampling; and (iv) academic study and processing for water column, beach environment, marsh peat and eel grass; provided further, that not less than $50,000 shall be expended to establish a program to collect and remove derelict gear; provided further, that not less than $60,000 shall be expended for the operation of the commercial fisheries commission established under
section 79 of chapter 179 of the acts of 2022; and provided further, that not less than $50,000 shall be expended for a boat for the harbormaster in the town of Saugus